Hotspot

EI144_pgp35

LSC · 37062-38025 · 964 bp

Back to Lepocinclis

Pi 0.315
MarkerSeek score 83.80
Primer available yes
Label EI144_pgp35

Feature Metadata

label_name
EI144_pgp35
feature_type
gene
region
LSC
length_bp
964
variable_sites
485
pi
0.315
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.97
markerseek_score
83.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
EI144_pgp35_p1 CCAGTTCGAATCCGGGTATC AAAGATTGTCCGCTTCCAGG 55.0 50.0 57.8 58.2 1656 bp 0.25 47.4
EI144_pgp35_p2 CCAGTTCGAATCCGGGTATC TCATTTGTTGGCGCTCCTAA 55.0 45.0 57.8 57.8 1845 bp 0.25 47.3
EI144_pgp35_p3 CCAGTTCGAATCCGGGTATC TTGTTGGCGCTCCTAATTCA 55.0 45.0 57.8 57.8 1841 bp 0.25 47.3
EI144_pgp35_p4 CAGTTCGAATCCGGGTATCG AAAGATTGTCCGCTTCCAGG 55.0 50.0 58.2 58.2 1655 bp 0.25 46.7
EI144_pgp35_p5 GTTCGAATCCGGGTATCGTC AAAGATTGTCCGCTTCCAGG 55.0 50.0 58.2 58.2 1653 bp 0.25 46.7