Hotspot

EI144_pgp24

LSC · 45650-46475 · 826 bp

Back to Lepocinclis

Pi 0.287

MarkerSeek score 82.80

Primer available yes

Label EI144_pgp24

Feature Metadata

label_name: EI144_pgp24
feature_type: gene
region: LSC
length_bp: 826
variable_sites: 367
pi: 0.287
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
EI144_pgp24_p1	TATACCACCTCCACTTGCCT	AGCTTGTGAGGTTTGGGAAG	50.0	50.0	57.7	58.0	1473 bp	0.25	47.8
EI144_pgp24_p2	TATACCACCTCCACTTGCCT	GAAGCTTGTGAGGTTTGGGA	50.0	50.0	57.7	58.0	1475 bp	0.25	47.7
EI144_pgp24_p3	TATACCACCTCCACTTGCCT	GCTTGTGAGGTTTGGGAAGA	50.0	50.0	57.7	58.0	1472 bp	0.25	47.7
EI144_pgp24_p4	TATACCACCTCCACTTGCCT	CTGCAGCTTGTGAAGTTTGG	50.0	50.0	57.7	57.9	1810 bp	0.25	46.9
EI144_pgp24_p5	TATACCACCTCCACTTGCCT	CGCGAAGGTGGAGATGTTAT	50.0	50.0	57.7	57.8	1862 bp	0.25	46.1