Hotspot

rpl16

LSC · 85393-86842 · 1450 bp

Back to Leplaea

Pi 0.0042

MarkerSeek score 50.20

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1450
variable_sites: 9
pi: 0.0042
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 50.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TGTAAAGTAAAGCAAAGGACGA	CCGCAATTCTACAGGGTTGA	36.4	50.0	56.0	57.9	1580-1597 bp	1.0	48.4
rpl16_p2	TGTAAAGTAAAGCAAAGGACGA	TCCGCAATTCTACAGGGTTG	36.4	50.0	56.0	57.9	1581-1598 bp	1.0	48.4
rpl16_p3	AAGTAAAGCAAAGGACGAAG	CCGCAATTCTACAGGGTTGA	40.0	50.0	53.9	57.9	1576-1593 bp	1.0	47.5
rpl16_p4	AAGTAAAGCAAAGGACGAAG	TCCGCAATTCTACAGGGTTG	40.0	50.0	53.9	57.9	1577-1594 bp	1.0	47.5
rpl16_p5	TCCAAGATTTCTTTCCTTTGGT	CCGCAATTCTACAGGGTTGA	36.4	50.0	55.8	57.9	1514-1535 bp	1.0	47.1