Hotspot

rbcL

LSC · 58663-60099 · 1437 bp

Back to Leplaea

Pi 0.00232

MarkerSeek score 60.20

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1437
variable_sites: 5
pi: 0.00232
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	TGAGTTGTAGGGAGGGACTT	GGGTTCGGCTCAATCCTTTT	50.0	50.0	57.3	58.5	1539 bp	1.0	70.9
rbcL_p2	AGACCTTGTTCTTGCGAGAA	GGGTTCGGCTCAATCCTTTT	45.0	50.0	57.1	58.5	1570 bp	1.0	69.3
rbcL_p3	TCATGAGTTGTAGGGAGGGA	GGGTTCGGCTCAATCCTTTT	50.0	50.0	57.1	58.5	1542 bp	1.0	69.1
rbcL_p4	CATGAGTTGTAGGGAGGGAC	GGGTTCGGCTCAATCCTTTT	55.0	50.0	57.0	58.5	1541 bp	1.0	68.7
rbcL_p5	CCTGTGAAGGGGTTTCATTCA	GGGTTCGGCTCAATCCTTTT	47.6	50.0	58.1	58.5	1610 bp	1.0	67.6