Hotspot

ndhA

SSC · 125033-127249 · 2217 bp

Back to Leplaea

Pi 0.00391

MarkerSeek score 59.80

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2217
variable_sites: 13
pi: 0.00391
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	GTTTGAGACAAGCGGGGAAT	ATGGGGGAAGTTGATCGTTG	50.0	50.0	58.5	57.9	2276-2282 bp	1.0	75.8
ndhA_p2	GTTTGAGACAAGCGGGGAAT	GGGGGAAGTTGATCGTTGAA	50.0	50.0	58.5	57.8	2274-2280 bp	1.0	75.3
ndhA_p3	GTTTGAGACAAGCGGGGAAT	GCCCACCGGGTTTTATCAAT	50.0	50.0	58.5	58.5	2374-2380 bp	1.0	72.6
ndhA_p4	GTTTGAGACAAGCGGGGAA	ATGGGGGAAGTTGATCGTTG	52.6	50.0	58.0	57.9	2276-2282 bp	1.0	71.4
ndhA_p5	GTTTGAGACAAGCGGGGAA	GGGGGAAGTTGATCGTTGAA	52.6	50.0	58.0	57.8	2274-2280 bp	1.0	70.8