Hotspot

clpP1

LSC · 74003-76080 · 2078 bp

Back to Leplaea

Pi 0.00289

MarkerSeek score 59.90

Primer available yes

Label clpP1

Feature Metadata

label_name: clpP1
feature_type: gene
region: LSC
length_bp: 2078
variable_sites: 9
pi: 0.00289
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP1_p1	TGCGTGAAATCACTCTTCTT	ACGCTTCCACATCAAAGTGA	40.0	45.0	55.3	57.7	2143-2153 bp	1.0	57.2
clpP1_p2	TTGCGTGAAATCACTCTTCT	ACGCTTCCACATCAAAGTGA	40.0	45.0	55.3	57.7	2144-2154 bp	1.0	57.2
clpP1_p3	TGCGTGAAATCACTCTTCTTT	ACGCTTCCACATCAAAGTGA	38.1	45.0	56.1	57.7	2143-2153 bp	1.0	55.6
clpP1_p4	TTGCGTGAAATCACTCTTCTT	ACGCTTCCACATCAAAGTGA	38.1	45.0	56.1	57.7	2144-2154 bp	1.0	55.6
clpP1_p5	TTTGCGTGAAATCACTCTTCT	ACGCTTCCACATCAAAGTGA	38.1	45.0	56.1	57.7	2145-2155 bp	1.0	55.6