Hotspot

ccsA-ndhD

SSC · 120612-120932 · 321 bp

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Pi 0.0232

MarkerSeek score 50.60

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 321
variable_sites: 11
pi: 0.0232
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 50.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TCTGCAATTGTGGCTTCTCT	AGTGGACAAGGTCGAAGCTA	45.0	50.0	57.4	58.4	460-466 bp	1.0	75.6
ccsA-ndhD_p2	GGCTTCTCTCGGCTTTCTTA	AGTGGACAAGGTCGAAGCTA	50.0	50.0	57.3	58.4	449-455 bp	1.0	74.8
ccsA-ndhD_p3	TGCAATTGTGGCTTCTCTCG	AGTGGACAAGGTCGAAGCTA	50.0	50.0	58.8	58.4	458-464 bp	1.0	73.4
ccsA-ndhD_p4	TCTGCAATTGTGGCTTCTCT	TCCGGATTTCGTTCTCTCAC	45.0	50.0	57.4	57.4	484-490 bp	1.0	73.2
ccsA-ndhD_p5	TCTGCAATTGTGGCTTCTCT	CCGGATTTCGTTCTCTCACT	45.0	50.0	57.4	57.3	483-489 bp	1.0	73.1