Hotspot

psbH-petB

LSC · 78877-78989 · 113 bp

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Pi 0.0118

MarkerSeek score 60.00

Primer available yes

Label psbH-petB

Feature Metadata

label_name: psbH-petB
feature_type: igs
region: LSC
length_bp: 113
variable_sites: 2
pi: 0.0118
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbH-petB_p1	GTCGCAATGGCTCTATTTGC	TCTCAAGACGACAGACCCAT	50.0	50.0	57.9	58.1	269-275 bp	1.0	79.1
psbH-petB_p2	CTGGATGGGGAACTACTCCT	TCTCAAGACGACAGACCCAT	55.0	50.0	57.8	58.1	298-304 bp	1.0	78.5
psbH-petB_p3	GTCGCAATGGCTCTATTTGC	AGACGACAGACCCATTCTCT	50.0	50.0	57.9	57.8	264-270 bp	1.0	77.9
psbH-petB_p4	CTTTGATGGGTGTCGCAATG	TCTCAAGACGACAGACCCAT	50.0	50.0	57.7	58.1	280-286 bp	1.0	77.8
psbH-petB_p5	GTCGCAATGGCTCTATTTGC	GACGACAGACCCATTCTCTG	50.0	55.0	57.9	57.7	263-269 bp	1.0	77.4