Hotspot

psbT-psbN

LSC · 78354-78418 · 65 bp

Back to Leplaea

Pi 0.0205

MarkerSeek score 51.90

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 65
variable_sites: 2
pi: 0.0205
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	TCGAGAACCGCCTAAAGTTC	GCAACCCTCTCAACAGCTAA	50.0	50.0	57.6	57.8	148 bp	1.0	76.7
psbT-psbN_p2	GAGAACCGCCTAAAGTTCCA	GCAACCCTCTCAACAGCTAA	50.0	50.0	57.5	57.8	146 bp	1.0	75.9
psbT-psbN_p3	TCGAGAACCGCCTAAAGTTC	GATCCATTCGAGGAACACGG	50.0	55.0	57.6	58.4	126 bp	1.0	75.0
psbT-psbN_p4	TCGAGAACCGCCTAAAGTTC	TTCGAGGAACACGGGGATTA	50.0	50.0	57.6	58.4	120 bp	1.0	74.9
psbT-psbN_p5	CGAGAACCGCCTAAAGTTCC	GCAACCCTCTCAACAGCTAA	55.0	50.0	58.6	57.8	147 bp	1.0	74.5