Hotspot

psaA-ycf3

LSC · 44448-45111 · 664 bp

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Pi 0.00603

MarkerSeek score 50.20

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 664
variable_sites: 6
pi: 0.00603
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	AGCGCAGAATTGGTTGAAGA	50.0	45.0	57.9	58.1	783-784 bp	1.0	79.0
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	ATTGGTTGAAGATCACGGGG	50.0	50.0	57.9	57.9	775-776 bp	1.0	78.8
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	AGCTATTGCGCTTACTCCTG	50.0	50.0	57.9	57.8	816-817 bp	1.0	77.9
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	TGAAGCGCAGAATTGGTTGA	50.0	45.0	57.9	58.4	786-787 bp	1.0	76.6
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	TTGAAGCGCAGAATTGGTTG	50.0	45.0	57.9	57.6	787-788 bp	1.0	76.4