Hotspot

trnM-rps14

LSC · 39357-39518 · 162 bp

Back to Leplaea

Pi 0.00823

MarkerSeek score 52.90

Primer available yes

Label trnM-rps14

Feature Metadata

label_name: trnM-rps14
feature_type: igs
region: LSC
length_bp: 162
variable_sites: 2
pi: 0.00823
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-rps14_p1	AAGGTTATGAGCCTTGCGAG	CGTTGTTTTTCGACCGGAAG	50.0	50.0	58.0	58.0	325 bp	1.0	80.1
trnM-rps14_p2	TTTGAACCCGTGACCTCAAG	CGTTGTTTTTCGACCGGAAG	50.0	50.0	58.0	58.0	342 bp	1.0	80.1
trnM-rps14_p3	GATTTGAACCCGTGACCTCA	CGTTGTTTTTCGACCGGAAG	50.0	50.0	57.8	58.0	344 bp	1.0	78.9
trnM-rps14_p4	GTTATGAGCCTTGCGAGCTA	CGTTGTTTTTCGACCGGAAG	50.0	50.0	57.8	58.0	322 bp	1.0	78.6
trnM-rps14_p5	TTATGAGCCTTGCGAGCTAC	CGTTGTTTTTCGACCGGAAG	50.0	50.0	57.8	58.0	321 bp	1.0	78.6