Hotspot

trnS-trnG

LSC · 9191-9983 · 793 bp

Back to Leplaea

Pi 0.00338

MarkerSeek score 58.40

Primer available yes

Label trnS-trnG

Feature Metadata

label_name: trnS-trnG
feature_type: igs
region: LSC
length_bp: 793
variable_sites: 4
pi: 0.00338
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-trnG_p1	CCGACGCTTTAATCCACTCA	AGGGGATTAATCGAACGAATCA	50.0	40.9	58.0	57.3	872 bp	1.0	59.3
trnS-trnG_p2	CCGACGCTTTAATCCACTCA	CGAACGAATCACACTTTTACCA	50.0	40.9	58.0	57.3	861 bp	1.0	59.0
trnS-trnG_p3	CCGACGCTTTAATCCACTCA	GGGGATTAATCGAACGAATCAC	50.0	45.5	58.0	57.3	871 bp	1.0	59.0
trnS-trnG_p4	ACGGAAAGAGAGGGATTCGA	AGGGGATTAATCGAACGAATCA	50.0	40.9	58.2	57.3	930 bp	1.0	58.1
trnS-trnG_p5	ACGGAAAGAGAGGGATTCGA	CGAACGAATCACACTTTTACCA	50.0	40.9	58.2	57.3	919 bp	1.0	57.8