Hotspot

rps16-trnQ

LSC · 6431-7841 · 1411 bp

Back to Leplaea

Pi 0.00287

MarkerSeek score 52.70

Primer available yes

Label rps16-trnQ

Feature Metadata

label_name: rps16-trnQ
feature_type: igs
region: LSC
length_bp: 1411
variable_sites: 6
pi: 0.00287
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 52.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1468-1484 bp	1.0	79.5
rps16-trnQ_p2	CATAGCGGATCATGGCCTTC	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.6	58.1	1488-1504 bp	1.0	76.1
rps16-trnQ_p3	AAGTCGCACGTTGCTTTCTA	GGGTTTTGGTCCCGGTATTC	45.0	55.0	58.1	58.5	1489-1505 bp	1.0	75.8
rps16-trnQ_p4	CGGATCATGGCCTTCAAGTC	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.7	58.1	1483-1499 bp	1.0	75.0
rps16-trnQ_p5	GGATCATGGCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.7	58.1	1482-1498 bp	1.0	75.0