Hotspot

rps16

LSC · 2428-3489 · 1062 bp

Back to Lepisorus

Pi 0.0788

MarkerSeek score 83.70

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 139
pi: 0.0788
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 83.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	GCTTGCTTAGCCGCATATTG	ATGCGACGTATGCAGCTAAA	50.0	45.0	58.0	58.1	1022-1531 bp	0.5	66.8
rps16_p2	CCCCTGCTTTTCCAACTTCT	ATGCGACGTATGCAGCTAAA	50.0	45.0	58.0	58.1	1453 bp	0.25	49.5
rps16_p3	CCCCTGCTTTTCCAACTTCT	TCTAGGTTACGAAGGCACCA	50.0	50.0	58.0	58.1	1719 bp	0.25	49.4
rps16_p4	CCCCTGCTTTTCCAACTTCT	GAGCGTCATATTGGCAAACG	50.0	50.0	58.0	57.9	1811 bp	0.25	49.3
rps16_p5	CCCCTGCTTTTCCAACTTCT	CCCTAGGATACCGACCCTTT	50.0	55.0	58.0	57.9	1738 bp	0.25	49.2