Hotspot

rps12-rrn16

IRa · 140936-145354 · 4419 bp

Back to Lepisorus

Pi 0.12

MarkerSeek score 77.60

Primer available yes

Label rps12-rrn16

Feature Metadata

label_name: rps12-rrn16
feature_type: igs
region: IRa
length_bp: 4419
variable_sites: 426
pi: 0.12
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.43
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-rrn16_p1	AGTACTTCCCCCAGTCGAAA	ACTGCCGAGTCATGTCAATC	50.0	50.0	58.0	58.0	531 bp	0.25	49.7
rps12-rrn16_p2	CGGAGTATTTGGCAAGCTCT	ACTGCCGAGTCATGTCAATC	50.0	50.0	58.0	58.0	649 bp	0.25	49.6
rps12-rrn16_p3	GAAGGTCGTCGAGTCTTGAC	ACTGCCGAGTCATGTCAATC	55.0	50.0	58.0	58.0	607 bp	0.25	49.6
rps12-rrn16_p4	GACTTGAAGGTCGTCGAGTC	ACTGCCGAGTCATGTCAATC	55.0	50.0	58.0	58.0	612 bp	0.25	49.6
rps12-rrn16_p5	AGTACTTCCCCCAGTCGAAA	ATACGCCTAGAGTGACGTCA	50.0	50.0	58.0	58.0	469 bp	0.25	49.6