Hotspot

rrn16-rps12

IRb · 92738-97156 · 4419 bp

Back to Lepisorus

Pi 0.118

MarkerSeek score 77.40

Primer available yes

Label rrn16-rps12

Feature Metadata

label_name: rrn16-rps12
feature_type: igs
region: IRb
length_bp: 4419
variable_sites: 422
pi: 0.118
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.43
markerseek_score: 77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn16-rps12_p1	ACTGCCGAGTCATGTCAATC	AGTACTTCCCCCAGTCGAAA	50.0	50.0	58.0	58.0	531 bp	0.25	49.7
rrn16-rps12_p2	ACTGCCGAGTCATGTCAATC	CGGAGTATTTGGCAAGCTCT	50.0	50.0	58.0	58.0	649 bp	0.25	49.6
rrn16-rps12_p3	ACTGCCGAGTCATGTCAATC	GAAGGTCGTCGAGTCTTGAC	50.0	55.0	58.0	58.0	607 bp	0.25	49.6
rrn16-rps12_p4	ACTGCCGAGTCATGTCAATC	GACTTGAAGGTCGTCGAGTC	50.0	55.0	58.0	58.0	612 bp	0.25	49.6
rrn16-rps12_p5	ATACGCCTAGAGTGACGTCA	AGTACTTCCCCCAGTCGAAA	50.0	50.0	58.0	58.0	469 bp	0.25	49.6