Hotspot

trnT-GGU-trnfM-CAU

LSC · 34468-34946 · 479 bp

Back to Lepisorus

Pi 0.0699

MarkerSeek score 83.20

Primer available yes

Label trnT-GGU-trnfM-CAU

Feature Metadata

label_name: trnT-GGU-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 479
variable_sites: 63
pi: 0.0699
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-trnfM-CAU_p1	TTAGCCCCTTGTCGGATTTG	CAGGTTCAAATCCTGTCCCC	50.0	55.0	57.9	58.2	573-583 bp	1.0	86.6
trnT-GGU-trnfM-CAU_p2	TTAGCCCCTTGTCGGATTTG	GTAGCTCGCAAGGCTCATAA	50.0	50.0	57.9	57.8	604-614 bp	1.0	86.0
trnT-GGU-trnfM-CAU_p3	TTAGCCCCTTGTCGGATTTG	TAGCTCGCAAGGCTCATAAC	50.0	50.0	57.9	57.8	603-613 bp	1.0	86.0
trnT-GGU-trnfM-CAU_p4	TTAGCCCCTTGTCGGATTTG	TGTTGGGGGCATCAACTAAG	50.0	50.0	57.9	57.8	641-651 bp	1.0	85.9
trnT-GGU-trnfM-CAU_p5	ACGACTTACGCCTTACCATG	CAGGTTCAAATCCTGTCCCC	50.0	55.0	57.7	58.2	548-558 bp	1.0	85.3