Hotspot

psbD-trnT-GGU

LSC · 33003-34395 · 1393 bp

Back to Lepisorus

Pi 0.0636
MarkerSeek score 83.10
Primer available yes
Label psbD-trnT-GGU

Feature Metadata

label_name
psbD-trnT-GGU
feature_type
igs
region
LSC
length_bp
1393
variable_sites
165
pi
0.0636
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
83.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbD-trnT-GGU_p1 TTTTCCGATGGCAATGGTCA AACTCAGGGGTAGAGTGACG 45.0 55.0 58.1 58.5 1478-1503 bp 1.0 85.2
psbD-trnT-GGU_p2 TTTCCGATGGCAATGGTCAT AACTCAGGGGTAGAGTGACG 45.0 55.0 57.9 58.5 1477-1502 bp 1.0 84.7
psbD-trnT-GGU_p3 TTTTCCGATGGCAATGGTCA GCCATGGTAAGGCGTAAGTC 45.0 55.0 58.1 58.7 1459-1484 bp 1.0 83.2
psbD-trnT-GGU_p4 TTTCCGATGGCAATGGTCAT GCCATGGTAAGGCGTAAGTC 45.0 55.0 57.9 58.7 1458-1483 bp 1.0 82.7
psbD-trnT-GGU_p5 TTTTCCGATGGCAATGGTCA GGTTCAAATCCGACAAGGGG 45.0 55.0 58.1 58.8 1436-1461 bp 1.0 82.2