Hotspot

psbZ-trnS-UGA

LSC · 29772-30172 · 401 bp

Back to Lepisorus

Pi 0.059

MarkerSeek score 80.90

Primer available yes

Label psbZ-trnS-UGA

Feature Metadata

label_name: psbZ-trnS-UGA
feature_type: igs
region: LSC
length_bp: 401
variable_sites: 43
pi: 0.059
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnS-UGA_p1	AGCCAATTGGAATGCGATAGT	CCGCTCGACCATCTCTCC	42.9	66.7	58.1	59.3	376-451 bp	1.0	57.3
psbZ-trnS-UGA_p2	GCCAATTGGAATGCGATAGTC	CCGCTCGACCATCTCTCC	47.6	66.7	57.7	59.3	375-450 bp	1.0	55.6
psbZ-trnS-UGA_p3	AAAGCCAATTGGAATGCGAT	CCGCTCGACCATCTCTCC	40.0	66.7	56.6	59.3	378-453 bp	1.0	55.0
psbZ-trnS-UGA_p4	GCCAATTGGAATGCGATAGT	CCGCTCGACCATCTCTCC	45.0	66.7	56.5	59.3	375-450 bp	1.0	54.2
psbZ-trnS-UGA_p5	AGCCAATTGGAATGCGATAG	CCGCTCGACCATCTCTCC	45.0	66.7	56.3	59.3	376-451 bp	1.0	52.0