Hotspot

trnC-GCA-trnG-GCC

LSC · 28750-29234 · 485 bp

Back to Lepisorus

Pi 0.0671
MarkerSeek score 81.20
Primer available yes
Label trnC-GCA-trnG-GCC

Feature Metadata

label_name
trnC-GCA-trnG-GCC
feature_type
igs
region
LSC
length_bp
485
variable_sites
59
pi
0.0671
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-trnG-GCC_p1 GATTTGCAGTCCTCCGTCTT GTTCGATTCCCGCTATCCG 50.0 57.9 57.9 58.2 540-563 bp 1.0 78.8
trnC-GCA-trnG-GCC_p2 GATTTGCAGTCCTCCGTCTT GGGTTCGATTCCCGCTATC 50.0 57.9 57.9 57.8 542-565 bp 1.0 78.7
trnC-GCA-trnG-GCC_p3 GATTTGCAGTCCTCCGTCTT GGTTCGATTCCCGCTATCC 50.0 57.9 57.9 57.8 541-564 bp 1.0 78.7
trnC-GCA-trnG-GCC_p4 TCCGTCTTACCGCTTGACTA GTTCGATTCCCGCTATCCG 50.0 57.9 58.2 58.2 528-551 bp 1.0 78.3
trnC-GCA-trnG-GCC_p5 TCCGTCTTACCGCTTGACTA GGTTCGATTCCCGCTATCC 50.0 57.9 58.2 57.8 529-552 bp 1.0 78.1