Hotspot

petN-trnC-GCA

LSC · 28221-28677 · 457 bp

Back to Lepisorus

Pi 0.0496

MarkerSeek score 81.20

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: LSC
length_bp: 457
variable_sites: 43
pi: 0.0496
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	GAGCTCCCCCAGATAATTGC	TGCAGGCGAAGACCTATCTA	55.0	50.0	58.1	57.9	1354-1379 bp	0.75	77.0
petN-trnC-GCA_p2	GTTTTTCATTCCGGGTGCAG	TGCAGGCGAAGACCTATCTA	50.0	50.0	57.9	57.9	1562-1569 bp	0.5	64.3
petN-trnC-GCA_p3	CTGTTTTTCATTCCGGGTGC	TGCAGGCGAAGACCTATCTA	50.0	50.0	57.9	57.9	1571 bp	0.25	48.9
petN-trnC-GCA_p4	GAGTTAACGGAGGTGTGACC	TGCAGGCGAAGACCTATCTA	55.0	50.0	57.9	57.9	1519 bp	0.25	48.9
petN-trnC-GCA_p5	TCCCTGTTTTTCATTCCGGG	TGCAGGCGAAGACCTATCTA	50.0	50.0	58.1	57.9	1574 bp	0.25	48.8