Hotspot

rpoB-trnD-GUC

LSC · 25432-26136 · 705 bp

Back to Lepisorus

Pi 0.0579

MarkerSeek score 85.20

Primer available yes

Label rpoB-trnD-GUC

Feature Metadata

label_name: rpoB-trnD-GUC
feature_type: igs
region: LSC
length_bp: 705
variable_sites: 75
pi: 0.0579
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 85.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnD-GUC_p1	CAAGTTCTGGTAGTACGGGC	GAGTTCATCGGGATTGACGG	55.0	55.0	58.0	58.4	773-809 bp	1.0	88.2
rpoB-trnD-GUC_p2	CAAGTTCTGGTAGTACGGGC	GGAGTTCATCGGGATTGACG	55.0	55.0	58.0	58.4	774-810 bp	1.0	88.2
rpoB-trnD-GUC_p3	GCAAGTTCTGGTAGTACGGG	GAGTTCATCGGGATTGACGG	55.0	55.0	58.0	58.4	774-810 bp	1.0	88.2
rpoB-trnD-GUC_p4	GCAAGTTCTGGTAGTACGGG	GGAGTTCATCGGGATTGACG	55.0	55.0	58.0	58.4	775-811 bp	1.0	88.2
rpoB-trnD-GUC_p5	AGTTCTGGTAGTACGGGCAT	GAGTTCATCGGGATTGACGG	50.0	55.0	58.1	58.4	771-807 bp	1.0	87.1