Hotspot

atpH-atpI

LSC · 12349-13070 · 722 bp

Back to Lepisorus

Pi 0.0594

MarkerSeek score 81.50

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 722
variable_sites: 79
pi: 0.0594
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	TCCCTCCTGGAAAGTTTTGC	CATTCAGTTACCCCACGGAG	50.0	55.0	58.0	58.0	1690-1729 bp	1.0	87.9
atpH-atpI_p2	TCCCTCCTGGAAAGTTTTGC	AGGTGCCAGAAATCTGCAAA	50.0	45.0	58.0	58.0	1882-1921 bp	0.75	75.2
atpH-atpI_p3	GAGGGAGTAGCGATCTCACT	AGGTGCCAGAAATCTGCAAA	55.0	45.0	58.0	58.0	1921-1960 bp	0.75	75.0
atpH-atpI_p4	ACACCCCCTTTCCGTAGTAA	AGGTGCCAGAAATCTGCAAA	50.0	45.0	58.0	58.0	2034-2073 bp	0.75	74.8
atpH-atpI_p5	TCCCTCCTGGAAAGTTTTGC	GCAAGTAGGGGTCTTCGAAG	50.0	55.0	58.0	58.0	1973 bp	0.25	49.9