Hotspot

matK-rps16

LSC · 1760-2427 · 668 bp

Back to Lepisorus

Pi 0.0626

MarkerSeek score 83.70

Primer available yes

Label matK-rps16

Feature Metadata

label_name: matK-rps16
feature_type: igs
region: LSC
length_bp: 668
variable_sites: 76
pi: 0.0626
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 83.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-rps16_p1	ACGCTATCGATTGAACGCTT	ATTTCGAAAGGGGCAAGGTT	45.0	45.0	58.1	58.0	914-949 bp	0.75	79.0
matK-rps16_p2	TCGATTGAACGCTTTGTTGC	ATTTCGAAAGGGGCAAGGTT	45.0	45.0	58.0	58.0	908-943 bp	0.5	66.8
matK-rps16_p3	TCGATTGAACGCTTTGTTGC	AGTAGCTCAATCAACAGCGG	45.0	50.0	58.0	58.0	939-974 bp	0.5	66.8
matK-rps16_p4	AGCTAGTTGACGCAGACAAG	ATTTCGAAAGGGGCAAGGTT	50.0	45.0	57.9	58.0	1067-1091 bp	0.5	64.7
matK-rps16_p5	AGCTAGTTGACGCAGACAAG	AGTAGCTCAATCAACAGCGG	50.0	50.0	57.9	58.0	1122 bp	0.25	49.3