Hotspot

ycf1

SSC · 123092-128416 · 5325 bp

Back to Lepidium

Pi 0.0286

MarkerSeek score 68.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5325
variable_sites: 634
pi: 0.0286
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCCATCGCAATTGAAAACGC	GAAACCGTGGGTCATACCAA	45.0	50.0	58.0	57.8	344-359 bp	0.933333	87.0
ycf1_p2	TCCATCGCAATTGAAAACGC	GTACAGGGGAAATTCGGCAT	45.0	50.0	58.0	57.9	654 bp	0.866667	82.1
ycf1_p3	GGGCCAAAGAAGGGATTTGA	GTACAGGGGAAATTCGGCAT	50.0	50.0	58.1	57.9	1220 bp	0.8	78.7
ycf1_p4	TCCATCGCAATTGAAAACGC	ACAGGTCTTGCCAACCAAAA	45.0	45.0	58.0	58.1	493 bp	0.333333	58.5
ycf1_p5	GGGCCAAAGAAGGGATTTGA	ACAGGTCTTGCCAACCAAAA	50.0	45.0	58.1	58.1	1059 bp	0.266667	51.7