Hotspot

rpl32-trnL-CUA

SSC · 112535-113458 · 924 bp

Back to Lepidium

Pi 0.0548
MarkerSeek score 78.90
Primer available yes
Label rpl32-trnL-CUA

Feature Metadata

label_name
rpl32-trnL-CUA
feature_type
igs
region
SSC
length_bp
924
variable_sites
178
pi
0.0548
unique_haplotype_count
14
primer_available
yes
alignment_reliability
0.99
markerseek_score
78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-CUA_p1 GGACTCTCGTACGGATTTGA TGCAGACTGAGGCATTTTGT 50.0 45.0 56.8 58.0 1908-1909 bp 0.133333 36.4
rpl32-trnL-CUA_p2 AGGACTCTCGTACGGATTTG TGCAGACTGAGGCATTTTGT 50.0 45.0 56.8 58.0 1909-1910 bp 0.133333 36.3
rpl32-trnL-CUA_p3 GGACTCTCGTACGGATTTGA TGAAAAAGCCCACGAAAGGA 50.0 45.0 56.8 57.9 1779-1780 bp 0.133333 36.1
rpl32-trnL-CUA_p4 GGACTCTCGTACGGATTTGA TAACCACTGGGACTGGAGAG 50.0 55.0 56.8 58.1 1941-1942 bp 0.133333 36.0
rpl32-trnL-CUA_p5 AGGACTCTCGTACGGATTTG TGAAAAAGCCCACGAAAGGA 50.0 45.0 56.8 57.9 1780-1781 bp 0.133333 36.0