Hotspot

ndhF-rpl32

SSC · 111531-112375 · 845 bp

Back to Lepidium

Pi 0.0398
MarkerSeek score 79.50
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
845
variable_sites
134
pi
0.0398
unique_haplotype_count
14
primer_available
yes
alignment_reliability
0.98
markerseek_score
79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TCCATGAATATTGATATGTATGTTCCA ACGAATACGCTTTTTCGAGA 29.6 40.0 56.7 55.5 903-949 bp 1.0 52.0
ndhF-rpl32_p2 CCATGAATATTGATATGTATGTTCCA ACGAATACGCTTTTTCGAGA 30.8 40.0 55.5 55.5 902-948 bp 1.0 52.0
ndhF-rpl32_p3 TCCATGAATATTGATATGTATGTTCCA ACGAATACGCTTTTTCGAGAT 29.6 38.1 56.7 56.1 903-949 bp 1.0 52.0
ndhF-rpl32_p4 TCCATGAATATTGATATGTATGTTCC ACGAATACGCTTTTTCGAGA 30.8 40.0 55.3 55.5 903-949 bp 1.0 52.0
ndhF-rpl32_p5 CCATGAATATTGATATGTATGTTCCA ACGAATACGCTTTTTCGAGAT 30.8 38.1 55.5 56.1 902-948 bp 1.0 52.0