Hotspot

petA-psbJ

LSC · 61816-62711 · 896 bp

Back to Lepidium

Pi 0.0304

MarkerSeek score 80.10

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 896
variable_sites: 119
pi: 0.0304
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	AGAAACAGTTTGAGAAGGTTCA	CAGGATTAGGTTCATCCCTGT	36.4	47.6	55.8	56.8	963-1019 bp	0.933333	49.3
petA-psbJ_p2	AGAAACAGTTTGAGAAGGTTCA	TCAGGATTAGGTTCATCCCTGT	36.4	45.5	55.8	58.3	964-1020 bp	0.933333	49.3
petA-psbJ_p3	AGAAACAGTTTGAGAAGGTTCA	AGCTGGTATTCTTGTGATTGGT	36.4	40.9	55.8	57.7	1016-1072 bp	0.933333	49.3
petA-psbJ_p4	AAGAAACAGTTTGAGAAGGTTCA	CAGGATTAGGTTCATCCCTGT	34.8	47.6	56.5	56.8	964-1020 bp	0.933333	49.3
petA-psbJ_p5	AGAAACAGTTTGAGAAGGTTCAA	CAGGATTAGGTTCATCCCTGT	34.8	47.6	56.5	56.8	963-1019 bp	0.933333	49.3