Hotspot

psbZ-trnG-GGC

LSC · 35462-35940 · 479 bp

Back to Lepidium

Pi 0.032

MarkerSeek score 78.90

Primer available yes

Label psbZ-trnG-GGC

Feature Metadata

label_name: psbZ-trnG-GGC
feature_type: igs
region: LSC
length_bp: 479
variable_sites: 66
pi: 0.032
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GGC_p1	TCCTGATGGTTGGTCGAGTA	CGGATAGCGGGAATCGAAC	50.0	57.9	57.8	58.2	637-681 bp	1.0	80.7
psbZ-trnG-GGC_p2	TCCTGATGGTTGGTCGAGTA	GATAGCGGGAATCGAACCC	50.0	57.9	57.8	57.8	635-679 bp	1.0	80.5
psbZ-trnG-GGC_p3	TCCTGATGGTTGGTCGAGTA	GGATAGCGGGAATCGAACC	50.0	57.9	57.8	57.8	636-680 bp	1.0	80.5
psbZ-trnG-GGC_p4	CCGTTGTATTTGCGTCTCCT	CGGATAGCGGGAATCGAAC	50.0	57.9	58.3	58.2	653-697 bp	1.0	80.3
psbZ-trnG-GGC_p5	CCGTTGTATTTGCGTCTCCT	GATAGCGGGAATCGAACCC	50.0	57.9	58.3	57.8	651-695 bp	1.0	80.1