Hotspot

trnD-GAC-trnY-UAC

LSC · 29594-30010 · 417 bp

Back to Lepidium

Pi 0.0444
MarkerSeek score 72.30
Primer available yes
Label trnD-GAC-trnY-UAC

Feature Metadata

label_name
trnD-GAC-trnY-UAC
feature_type
igs
region
LSC
length_bp
417
variable_sites
70
pi
0.0444
unique_haplotype_count
12
primer_available
yes
alignment_reliability
1.00
markerseek_score
72.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GAC-trnY-UAC_p1 ACCAATTGAACTACAATCCC TGGCAATATGTCTACGCTGG 40.0 50.0 53.1 57.8 477-511 bp 0.866667 46.2
trnD-GAC-trnY-UAC_p2 ACCAATTGAACTACAATCCC GGCAATATGTCTACGCTGGT 40.0 50.0 53.1 57.8 476-510 bp 0.866667 46.2
trnD-GAC-trnY-UAC_p3 ACCAATTGAACTACAATCCC GGGGACGGACTGTAAATTCG 40.0 55.0 53.1 58.4 498-532 bp 0.866667 46.1
trnD-GAC-trnY-UAC_p4 ACCAATTGAACTACAATCCC GGGACGGACTGTAAATTCGT 40.0 50.0 53.1 57.6 497-531 bp 0.866667 46.1
trnD-GAC-trnY-UAC_p5 ACCAATTGAACTACAATCCC GATTCTTCCTGGGTCGATGC 40.0 55.0 53.1 58.4 531-565 bp 0.866667 45.8