Hotspot

trnG-GGA-trnR-AGA

LSC · 9234-9387 · 154 bp

Back to Lepidium

Pi 0.0523

MarkerSeek score 76.10

Primer available yes

Label trnG-GGA-trnR-AGA

Feature Metadata

label_name: trnG-GGA-trnR-AGA
feature_type: igs
region: LSC
length_bp: 154
variable_sites: 25
pi: 0.0523
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GGA-trnR-AGA_p1	AGCCTTCCAAGCTAACGATG	TGATATTGCGTCCAATAGGA	50.0	40.0	58.0	53.9	258-292 bp	1.0	58.6
trnG-GGA-trnR-AGA_p2	ACCCTTAGCCTTCCAAGCTA	TGATATTGCGTCCAATAGGA	50.0	40.0	58.0	53.9	264-298 bp	1.0	58.4
trnG-GGA-trnR-AGA_p3	AAACCCTTAGCCTTCCAAGC	TGATATTGCGTCCAATAGGA	50.0	40.0	58.1	53.9	266-300 bp	1.0	58.0
trnG-GGA-trnR-AGA_p4	AGCCTTCCAAGCTAACGATG	TTGATATTGCGTCCAATAGGA	50.0	38.1	58.0	54.8	259-293 bp	1.0	57.6
trnG-GGA-trnR-AGA_p5	ACCCTTAGCCTTCCAAGCTA	TTGATATTGCGTCCAATAGGA	50.0	38.1	58.0	54.8	265-299 bp	1.0	57.4