primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-AAA-rps16_p1	trnK-AAA-rps16	1	CCTTTCAGGATCAGTCGTGG	ATCTTCGCAAGGTGGGTTTT	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.020360	4302	4952	889-1009	1.000000	91.8
trnK-AAA-rps16_p2	trnK-AAA-rps16	2	GCGGATCTGGACGAATTCTT	ATCTTCGCAAGGTGGGTTTT	20	20	50.0	45.0	58.1	58.0	3.1	0.0	0.063299	4302	4952	853-973	0.866667	86.2
trnK-AAA-rps16_p3	trnK-AAA-rps16	3	CCTTTCAGGATCAGTCGTGG	CCCAATGAGCCGTTTATCGA	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.067632	4302	4952	949-1069	0.866667	86.1
trnK-AAA-rps16_p4	trnK-AAA-rps16	4	CCTTTCAGGATCAGTCGTGG	TCCCAATGAGCCGTTTATCG	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.067632	4302	4952	950-1070	0.866667	86.1
trnK-AAA-rps16_p5	trnK-AAA-rps16	5	CCTTTCAGGATCAGTCGTGG	GGTTTGTTGCTGCCCTTTTT	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.052693	4302	4952	1417-1543	0.733333	80.0
rps16_p1	rps16	1	ACGAGTCTTTCACATCCTCT	TCAATTACACAAACTAGAGGAATGT	20	25	45.0	32.0	55.9	56.5	0.0	0.0	8.627837	4953	6070	1154-1226	1.000000	51.0
rps16_p2	rps16	2	AGAGGAGGAAAGCAAAGAGT	TCAATTACACAAACTAGAGGAATGT	20	25	45.0	32.0	56.1	56.5	0.0	0.0	8.469602	4953	6070	1200-1264	0.933333	47.0
rps16_p3	rps16	3	AAGAGGAGGAAAGCAAAGAGT	TCAATTACACAAACTAGAGGAATGT	21	25	42.9	32.0	56.8	56.5	0.0	0.0	8.688003	4953	6070	1201-1265	0.933333	47.0
rps16_p4	rps16	4	AGAGGAGGAAAGCAAAGAGT	TGAATCAATTACACAAACTAGAGGA	20	25	45.0	32.0	56.1	56.2	0.0	0.0	8.705638	4953	6070	1204-1268	0.933333	47.0
rps16_p5	rps16	5	AGAGGAGGAAAGCAAAGAGT	CAATTACACAAACTAGAGGAATGT	20	24	45.0	33.3	56.1	55.1	0.0	0.0	8.804892	4953	6070	1199-1263	0.933333	47.0
trnS-AGC-trnG-GGA_p1	trnS-AGC-trnG-GGA	1	CCAACGCTTTAGTCCACTCA	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	57.8	55.5	0.0	0.0	3.699660	7813	8444	685-730	1.000000	62.4
trnS-AGC-trnG-GGA_p2	trnS-AGC-trnG-GGA	2	AACGCTTTAGTCCACTCAGC	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.2	55.5	2.0	0.0	3.738818	7813	8444	683-728	1.000000	62.1
trnS-AGC-trnG-GGA_p3	trnS-AGC-trnG-GGA	3	CCAACGCTTTAGTCCACTCA	GAACGAATCACACTTTTACCACT	20	23	50.0	39.1	57.8	57.3	0.0	0.0	3.832376	7813	8444	687-732	1.000000	61.3
trnS-AGC-trnG-GGA_p4	trnS-AGC-trnG-GGA	4	AACGCTTTAGTCCACTCAGC	GAACGAATCACACTTTTACCACT	20	23	50.0	39.1	58.2	57.3	2.0	0.0	3.871534	7813	8444	685-730	1.000000	61.0
trnS-AGC-trnG-GGA_p5	trnS-AGC-trnG-GGA	5	CCAACGCTTTAGTCCACTCA	AACGAATCACACTTTTACCACT	20	22	50.0	36.4	57.8	56.2	0.0	0.0	3.940131	7813	8444	686-731	1.000000	60.5
trnG-GGA-trnR-AGA_p1	trnG-GGA-trnR-AGA	1	AGCCTTCCAAGCTAACGATG	TGATATTGCGTCCAATAGGA	20	20	50.0	40.0	58.0	53.9	0.0	3.0	4.105791	9234	9387	258-292	1.000000	58.6
trnG-GGA-trnR-AGA_p2	trnG-GGA-trnR-AGA	2	ACCCTTAGCCTTCCAAGCTA	TGATATTGCGTCCAATAGGA	20	20	50.0	40.0	58.0	53.9	0.0	3.0	4.109107	9234	9387	264-298	1.000000	58.4
trnG-GGA-trnR-AGA_p3	trnG-GGA-trnR-AGA	3	AAACCCTTAGCCTTCCAAGC	TGATATTGCGTCCAATAGGA	20	20	50.0	40.0	58.1	53.9	0.0	3.0	4.151221	9234	9387	266-300	1.000000	58.0
trnG-GGA-trnR-AGA_p4	trnG-GGA-trnR-AGA	4	AGCCTTCCAAGCTAACGATG	TTGATATTGCGTCCAATAGGA	20	21	50.0	38.1	58.0	54.8	0.0	3.0	4.230351	9234	9387	259-293	1.000000	57.6
trnG-GGA-trnR-AGA_p5	trnG-GGA-trnR-AGA	5	ACCCTTAGCCTTCCAAGCTA	TTGATATTGCGTCCAATAGGA	20	21	50.0	38.1	58.0	54.8	0.0	3.0	4.233668	9234	9387	265-299	1.000000	57.4
psbM-trnD-GAC_p1	psbM-trnD-GAC	1	GAGAATGAAGAGTGCAGTAGC	GTTCGAGCCCCGTCAGTC	21	18	47.6	66.7	56.4	60.1	0.0	0.0	6.682031	28495	29519	1069-1102	1.000000	52.0
psbM-trnD-GAC_p2	psbM-trnD-GAC	2	AGAATGAAGAGTGCAGTAGC	GTTCGAGCCCCGTCAGTC	20	18	45.0	66.7	55.2	60.1	0.0	0.0	6.944422	28495	29519	1068-1101	1.000000	52.0
psbM-trnD-GAC_p3	psbM-trnD-GAC	3	GAGAATGAAGAGTGCAGTAGC	GAGCACCGCCCTGTCAAG	21	18	47.6	66.7	56.4	60.7	0.0	0.0	7.299928	28495	29519	1100-1133	1.000000	52.0
psbM-trnD-GAC_p4	psbM-trnD-GAC	4	GAGAATGAAGAGTGCAGTAGC	GCCCTGTCAAGGCGGAAG	21	18	47.6	66.7	56.4	60.7	0.0	0.0	7.303855	28495	29519	1093-1126	1.000000	52.0
psbM-trnD-GAC_p5	psbM-trnD-GAC	5	AGAATGAAGAGTGCAGTAGC	GAGCACCGCCCTGTCAAG	20	18	45.0	66.7	55.2	60.7	0.0	0.0	7.562319	28495	29519	1099-1132	1.000000	52.0
trnD-GAC-trnY-UAC_p1	trnD-GAC-trnY-UAC	1	ACCAATTGAACTACAATCCC	TGGCAATATGTCTACGCTGG	20	20	40.0	50.0	53.1	57.8	7.1	0.0	5.107249	29594	30010	477-511	0.866667	46.2
trnD-GAC-trnY-UAC_p2	trnD-GAC-trnY-UAC	2	ACCAATTGAACTACAATCCC	GGCAATATGTCTACGCTGGT	20	20	40.0	50.0	53.1	57.8	7.1	0.0	5.108800	29594	30010	476-510	0.866667	46.2
trnD-GAC-trnY-UAC_p3	trnD-GAC-trnY-UAC	3	ACCAATTGAACTACAATCCC	GGGGACGGACTGTAAATTCG	20	20	40.0	55.0	53.1	58.4	7.1	0.0	5.219803	29594	30010	498-532	0.866667	46.1
trnD-GAC-trnY-UAC_p4	trnD-GAC-trnY-UAC	4	ACCAATTGAACTACAATCCC	GGGACGGACTGTAAATTCGT	20	20	40.0	50.0	53.1	57.6	7.1	0.0	5.243480	29594	30010	497-531	0.866667	46.1
trnD-GAC-trnY-UAC_p5	trnD-GAC-trnY-UAC	5	ACCAATTGAACTACAATCCC	GATTCTTCCTGGGTCGATGC	20	20	40.0	55.0	53.1	58.4	7.1	0.0	5.280760	29594	30010	531-565	0.866667	45.8
trnE-GAA-trnT-ACC_p1	trnE-GAA-trnT-ACC	1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	57.8	58.0	0.0	0.0	0.165150	30227	30970	579-967	1.000000	90.7
trnE-GAA-trnT-ACC_p2	trnE-GAA-trnT-ACC	2	TTTCGTAGTACCCTACCCCC	CCATGGCGTTACTCTACCAC	20	20	55.0	55.0	57.8	58.1	0.0	0.0	0.223247	30227	30970	558-946	1.000000	90.2
trnE-GAA-trnT-ACC_p3	trnE-GAA-trnT-ACC	3	TTTCGTAGTACCCTACCCCC	TGGCGTTACTCTACCACTGA	20	20	55.0	50.0	57.8	58.1	0.0	0.0	0.245401	30227	30970	555-943	1.000000	90.0
trnE-GAA-trnT-ACC_p4	trnE-GAA-trnT-ACC	4	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	58.3	58.0	0.0	0.0	0.269359	30227	30970	541-929	1.000000	89.8
trnE-GAA-trnT-ACC_p5	trnE-GAA-trnT-ACC	5	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.287509	30227	30970	520-908	1.000000	89.7
trnT-ACC-psbD_p1	trnT-ACC-psbD	1	GTGGTAGAGTAACGCCATGG	ACCTACAAAAACGAAGCGGT	20	20	55.0	45.0	58.1	58.0	7.6	0.0	0.106399	31043	32221	1123-1362	1.000000	89.7
trnT-ACC-psbD_p2	trnT-ACC-psbD	2	TCAGTGGTAGAGTAACGCCA	ACCTACAAAAACGAAGCGGT	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.128554	31043	32221	1126-1365	1.000000	89.5
trnT-ACC-psbD_p3	trnT-ACC-psbD	3	CCAAGACGGCCCTTTTAACT	ACCTACAAAAACGAAGCGGT	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.136694	31043	32221	1145-1384	1.000000	89.4
trnT-ACC-psbD_p4	trnT-ACC-psbD	4	ACGGCCCTTTTAACTCAGTG	ACCTACAAAAACGAAGCGGT	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.150122	31043	32221	1140-1379	1.000000	89.4
trnT-ACC-psbD_p5	trnT-ACC-psbD	5	GACGGCCCTTTTAACTCAGT	ACCTACAAAAACGAAGCGGT	20	20	50.0	45.0	57.8	58.0	0.0	0.0	0.222764	31043	32221	1141-1380	1.000000	88.8
psbZ-trnG-GGC_p1	psbZ-trnG-GGC	1	TCCTGATGGTTGGTCGAGTA	CGGATAGCGGGAATCGAAC	20	19	50.0	57.9	57.8	58.2	0.0	0.0	1.414896	35462	35940	637-681	1.000000	80.7
psbZ-trnG-GGC_p2	psbZ-trnG-GGC	2	TCCTGATGGTTGGTCGAGTA	GATAGCGGGAATCGAACCC	20	19	50.0	57.9	57.8	57.8	0.0	17.7	1.437832	35462	35940	635-679	1.000000	80.5
psbZ-trnG-GGC_p3	psbZ-trnG-GGC	3	TCCTGATGGTTGGTCGAGTA	GGATAGCGGGAATCGAACC	20	19	50.0	57.9	57.8	57.8	0.0	0.0	1.437832	35462	35940	636-680	1.000000	80.5
psbZ-trnG-GGC_p4	psbZ-trnG-GGC	4	CCGTTGTATTTGCGTCTCCT	CGGATAGCGGGAATCGAAC	20	19	50.0	57.9	58.3	58.2	0.0	0.0	1.467503	35462	35940	653-697	1.000000	80.3
psbZ-trnG-GGC_p5	psbZ-trnG-GGC	5	CCGTTGTATTTGCGTCTCCT	GATAGCGGGAATCGAACCC	20	19	50.0	57.9	58.3	57.8	0.0	17.7	1.490438	35462	35940	651-695	1.000000	80.1
trnF-UUC-ndhJ_p1	trnF-UUC-ndhJ	1	CCTCGTGTCACCAGTTCAAA	CCCGAAAGTTGGATAGGGTG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.090283	47747	48266	619-660	1.000000	91.3
trnF-UUC-ndhJ_p2	trnF-UUC-ndhJ	2	CCTCGTGTCACCAGTTCAAA	CCGAAAGTTGGATAGGGTGG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.090283	47747	48266	618-659	1.000000	91.3
trnF-UUC-ndhJ_p3	trnF-UUC-ndhJ	3	CCTCGTGTCACCAGTTCAAA	CCTAATGCCCGAAAGTTGGA	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.174679	47747	48266	626-667	1.000000	90.6
trnF-UUC-ndhJ_p4	trnF-UUC-ndhJ	4	CCTCGTGTCACCAGTTCAAA	TCCTAATGCCCGAAAGTTGG	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.174679	47747	48266	627-668	1.000000	90.6
trnF-UUC-ndhJ_p5	trnF-UUC-ndhJ	5	CCTCGTGTCACCAGTTCAAA	GAAAGTTGGATAGGGTGGCC	20	20	50.0	55.0	58.1	58.2	0.0	0.0	0.287928	47747	48266	616-657	1.000000	89.7
ndhC-trnV-GUA_p1	ndhC-trnV-GUA	1	TGCCAAAACAGGAATAGCACT	GGTTCGAATCCGTATAGCCC	21	20	42.9	55.0	58.1	57.9	0.0	0.0	1.200683	49952	50517	472-960	1.000000	81.8
ndhC-trnV-GUA_p2	ndhC-trnV-GUA	2	TGCCAAAACAGGAATAGCACT	CGGTTCGAATCCGTATAGCC	21	20	42.9	55.0	58.1	58.3	0.0	22.8	1.430064	49952	50517	473-961	1.000000	80.0
ndhC-trnV-GUA_p3	ndhC-trnV-GUA	3	TGCCAAAACAGGAATAGCAC	GGTTCGAATCCGTATAGCCC	20	20	45.0	55.0	56.6	57.9	0.0	0.0	1.470362	49952	50517	472-960	1.000000	79.7
ndhC-trnV-GUA_p4	ndhC-trnV-GUA	4	TGCCAAAACAGGAATAGCACT	CAGGTCTACGGTTCGAATCC	21	20	42.9	55.0	58.1	57.8	0.0	0.0	1.330010	49952	50517	481-969	0.933333	76.6
ndhC-trnV-GUA_p5	ndhC-trnV-GUA	5	TGCCAAAACAGGAATAGCACT	GCAGGTCTACGGTTCGAATC	21	20	42.9	55.0	58.1	58.4	0.0	0.0	1.567052	49952	50517	482-970	0.933333	74.7
petA-psbJ_p1	petA-psbJ	1	AGAAACAGTTTGAGAAGGTTCA	CAGGATTAGGTTCATCCCTGT	22	21	36.4	47.6	55.8	56.8	2.3	0.0	6.436629	61816	62711	963-1019	0.933333	49.3
petA-psbJ_p2	petA-psbJ	2	AGAAACAGTTTGAGAAGGTTCA	TCAGGATTAGGTTCATCCCTGT	22	22	36.4	45.5	55.8	58.3	2.3	0.0	6.477862	61816	62711	964-1020	0.933333	49.3
petA-psbJ_p3	petA-psbJ	3	AGAAACAGTTTGAGAAGGTTCA	AGCTGGTATTCTTGTGATTGGT	22	22	36.4	40.9	55.8	57.7	2.3	0.0	6.520987	61816	62711	1016-1072	0.933333	49.3
petA-psbJ_p4	petA-psbJ	4	AAGAAACAGTTTGAGAAGGTTCA	CAGGATTAGGTTCATCCCTGT	23	21	34.8	47.6	56.5	56.8	2.3	0.0	6.716745	61816	62711	964-1020	0.933333	49.3
petA-psbJ_p5	petA-psbJ	5	AGAAACAGTTTGAGAAGGTTCAA	CAGGATTAGGTTCATCCCTGT	23	21	34.8	47.6	56.5	56.8	2.3	0.0	6.716745	61816	62711	963-1019	0.933333	49.3
ndhF-rpl32_p1	ndhF-rpl32	1	TCCATGAATATTGATATGTATGTTCCA	ACGAATACGCTTTTTCGAGA	27	20	29.6	40.0	56.7	55.5	0.0	19.5	10.706462	111531	112375	903-949	1.000000	52.0
ndhF-rpl32_p2	ndhF-rpl32	2	CCATGAATATTGATATGTATGTTCCA	ACGAATACGCTTTTTCGAGA	26	20	30.8	40.0	55.5	55.5	0.0	19.5	10.945020	111531	112375	902-948	1.000000	52.0
ndhF-rpl32_p3	ndhF-rpl32	3	TCCATGAATATTGATATGTATGTTCCA	ACGAATACGCTTTTTCGAGAT	27	21	29.6	38.1	56.7	56.1	0.0	19.5	11.150403	111531	112375	903-949	1.000000	52.0
ndhF-rpl32_p4	ndhF-rpl32	4	TCCATGAATATTGATATGTATGTTCC	ACGAATACGCTTTTTCGAGA	26	20	30.8	40.0	55.3	55.5	0.0	19.5	11.174465	111531	112375	903-949	1.000000	52.0
ndhF-rpl32_p5	ndhF-rpl32	5	CCATGAATATTGATATGTATGTTCCA	ACGAATACGCTTTTTCGAGAT	26	21	30.8	38.1	55.5	56.1	0.0	19.5	11.388961	111531	112375	902-948	1.000000	52.0
rpl32-trnL-CUA_p1	rpl32-trnL-CUA	1	GGACTCTCGTACGGATTTGA	TGCAGACTGAGGCATTTTGT	20	20	50.0	45.0	56.8	58.0	16.5	6.7	1.234595	112535	113458	1908-1909	0.133333	36.4
rpl32-trnL-CUA_p2	rpl32-trnL-CUA	2	AGGACTCTCGTACGGATTTG	TGCAGACTGAGGCATTTTGT	20	20	50.0	45.0	56.8	58.0	16.5	6.7	1.243294	112535	113458	1909-1910	0.133333	36.3
rpl32-trnL-CUA_p3	rpl32-trnL-CUA	3	GGACTCTCGTACGGATTTGA	TGAAAAAGCCCACGAAAGGA	20	20	50.0	45.0	56.8	57.9	16.5	0.0	1.280119	112535	113458	1779-1780	0.133333	36.1
rpl32-trnL-CUA_p4	rpl32-trnL-CUA	4	GGACTCTCGTACGGATTTGA	TAACCACTGGGACTGGAGAG	20	20	50.0	55.0	56.8	58.1	16.5	0.0	1.281484	112535	113458	1941-1942	0.133333	36.0
rpl32-trnL-CUA_p5	rpl32-trnL-CUA	5	AGGACTCTCGTACGGATTTG	TGAAAAAGCCCACGAAAGGA	20	20	50.0	45.0	56.8	57.9	16.5	0.0	1.288817	112535	113458	1780-1781	0.133333	36.0
ycf1_p1	ycf1	1	TCCATCGCAATTGAAAACGC	GAAACCGTGGGTCATACCAA	20	20	45.0	50.0	58.0	57.8	10.3	0.0	0.212225	123856	123975	344-359	0.933333	87.0
ycf1_p2	ycf1	2	TCCATCGCAATTGAAAACGC	GTACAGGGGAAATTCGGCAT	20	20	45.0	50.0	58.0	57.9	10.3	0.0	0.076946	123856	123975	654-654	0.866667	82.1
ycf1_p3	ycf1	3	GGGCCAAAGAAGGGATTTGA	GTACAGGGGAAATTCGGCAT	20	20	50.0	50.0	58.1	57.9	4.4	0.0	0.115591	123856	123975	1220-1220	0.800000	78.7
ycf1_p4	ycf1	4	TCCATCGCAATTGAAAACGC	ACAGGTCTTGCCAACCAAAA	20	20	45.0	45.0	58.0	58.1	10.3	2.5	0.164861	123856	123975	493-493	0.333333	58.5
ycf1_p5	ycf1	5	GGGCCAAAGAAGGGATTTGA	ACAGGTCTTGCCAACCAAAA	20	20	50.0	45.0	58.1	58.1	4.4	2.5	0.203507	123856	123975	1059-1059	0.266667	51.7