Hotspot

ycf1

IRb · 104424-105530 · 1107 bp

Back to Lepechiniella

Pi 0.00365

MarkerSeek score 57.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 1107
variable_sites: 4
pi: 0.00365
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCCAGAAATCGCAATAGAAAAGA	AGGAGGTGGTCGTATCTCTT	34.8	50.0	56.3	57.2	1211-1223 bp	1.0	41.1
ycf1_p2	ACAGATGTCGTTTCTAGTCT	AGGAGGTGGTCGTATCTCTT	40.0	50.0	53.5	57.2	1284-1296 bp	1.0	41.0
ycf1_p3	AACAGATGTCGTTTCTAGTCT	AGGAGGTGGTCGTATCTCTT	38.1	50.0	54.4	57.2	1285-1297 bp	1.0	41.0
ycf1_p4	ACAGATGTCGTTTCTAGTCT	GGAGGTGGTCGTATCTCTTC	40.0	55.0	53.5	56.8	1283-1295 bp	1.0	41.0
ycf1_p5	AACAGATGTCGTTTCTAGTCT	GGAGGTGGTCGTATCTCTTC	38.1	55.0	54.4	56.8	1284-1296 bp	1.0	41.0