Hotspot

trnG-UCC

LSC · 9307-9354 · 48 bp

Back to Lepechiniella

Pi 0.0208

MarkerSeek score 64.50

Primer available yes

Label trnG-UCC

Feature Metadata

label_name: trnG-UCC
feature_type: tRNA
region: LSC
length_bp: 48
variable_sites: 1
pi: 0.0208
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC_p1	AAAGGAGCCGAATGAAACCA	TGTGAAAAATTTTCGGGAATTGT	45.0	30.4	57.7	56.1	202 bp	1.0	40.4
trnG-UCC_p2	AAGGAGCCGAATGAAACCAA	TGTGAAAAATTTTCGGGAATTGT	45.0	30.4	57.7	56.1	201 bp	1.0	40.4
trnG-UCC_p3	AGGAGCCGAATGAAACCAAA	TGTGAAAAATTTTCGGGAATTGT	45.0	30.4	57.7	56.1	200 bp	1.0	40.4
trnG-UCC_p4	GGAGCCGAATGAAACCAAAG	TGTGAAAAATTTTCGGGAATTGT	50.0	30.4	57.4	56.1	199 bp	1.0	40.4
trnG-UCC_p5	AGGAGCCGAATGAAACCAAA	GGATTGTATGTGAAAAATTTTCGGG	45.0	36.0	57.7	57.7	208 bp	1.0	40.4