Hotspot

rpl16

LSC · 77495-78977 · 1483 bp

Back to Lepechiniella

Pi 0.00882

MarkerSeek score 59.50

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1483
variable_sites: 13
pi: 0.00882
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TCAATGCAAACAAAGGACGA	ACTTATCTTTTCCTTCTATCCAA	40.0	30.4	56.2	52.8	1699-1706 bp	1.0	41.0
rpl16_p2	TGCAAACAAAGGACGAAGAA	ACTTATCTTTTCCTTCTATCCAA	40.0	30.4	56.1	52.8	1695-1702 bp	1.0	41.0
rpl16_p3	CAATGCAAACAAAGGACGAAGA	ACTTATCTTTTCCTTCTATCCAA	40.9	30.4	58.0	52.8	1698-1705 bp	1.0	41.0
rpl16_p4	TCAATGCAAACAAAGGACGAAG	ACTTATCTTTTCCTTCTATCCAA	40.9	30.4	58.0	52.8	1699-1706 bp	1.0	41.0
rpl16_p5	TCAATGCAAACAAAGGACGAA	ACTTATCTTTTCCTTCTATCCAA	38.1	30.4	56.9	52.8	1699-1706 bp	1.0	41.0