Hotspot

ndhD-psaC

SSC · 111859-111960 · 102 bp

Back to Lepechiniella

Pi 0.049

MarkerSeek score 70.30

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 102
variable_sites: 5
pi: 0.049
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	ACCAAGGAAAGTAATTCGTGGT	AAGAGATGTGAATCCGCCTG	40.9	50.0	57.9	57.7	223 bp	1.0	62.5
ndhD-psaC_p2	ACCAAGGAAAGTAATTCGTGGT	GAGATGTGAATCCGCCTGTC	40.9	55.0	57.9	58.4	221 bp	1.0	61.7
ndhD-psaC_p3	ACCAAGGAAAGTAATTCGTGGT	GCCTGTCCAACGGATTTCTT	40.9	50.0	57.9	58.5	208 bp	1.0	61.4
ndhD-psaC_p4	CCCGTGAATATTGGGAAAACA	AAGAGATGTGAATCCGCCTG	42.9	50.0	56.5	57.7	257 bp	1.0	58.9
ndhD-psaC_p5	ACCAAGGAAAGTAATTCGTGGT	AGAGATGTGAATCCGCCTGT	40.9	50.0	57.9	58.8	222 bp	1.0	58.6