Hotspot

ndhF-rpl32

SSC · 107730-108341 · 612 bp

Back to Lepechiniella

Pi 0.00493

MarkerSeek score 62.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 612
variable_sites: 3
pi: 0.00493
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CAAGGGTATGATCCACGCAT	TGTCCAATACCCCCTCCTTT	50.0	50.0	57.7	57.9	729-732 bp	1.0	78.1
ndhF-rpl32_p2	AAAAGAAGAAGCCCCACTCC	TGTCCAATACCCCCTCCTTT	50.0	50.0	57.7	57.9	776-779 bp	1.0	77.8
ndhF-rpl32_p3	AAGCAAGGGTATGATCCACG	TGTCCAATACCCCCTCCTTT	50.0	50.0	57.7	57.9	732-735 bp	1.0	77.6
ndhF-rpl32_p4	AAGAAGAAGCCCCACTCCTA	TGTCCAATACCCCCTCCTTT	50.0	50.0	57.7	57.9	774-777 bp	1.0	77.4
ndhF-rpl32_p5	CAAGGGTATGATCCACGCAT	GATGTCCAATACCCCCTCCT	50.0	55.0	57.7	58.2	731-734 bp	1.0	77.2