Hotspot

psaJ-rpl33

LSC · 63835-64185 · 351 bp

Back to Lepechiniella

Pi 0.0233

MarkerSeek score 69.10

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 351
variable_sites: 8
pi: 0.0233
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TCTCTGTGGCACCAGTTCTA	GACACAAGCGGTACATTCCA	50.0	50.0	58.0	58.2	513-521 bp	1.0	80.8
psaJ-rpl33_p2	ATGCGTTGACATTCCCCTTT	GACACAAGCGGTACATTCCA	45.0	50.0	58.1	58.2	432-440 bp	1.0	80.7
psaJ-rpl33_p3	TCTCTGTGGCACCAGTTCTA	ACACAAGCGGTACATTCCAA	50.0	45.0	58.0	57.7	512-520 bp	1.0	80.3
psaJ-rpl33_p4	ATGCGTTGACATTCCCCTTT	ACACAAGCGGTACATTCCAA	45.0	45.0	58.1	57.7	431-439 bp	1.0	80.2
psaJ-rpl33_p5	TATCTCTCTGTGGCACCAGT	GACACAAGCGGTACATTCCA	50.0	50.0	57.8	58.2	517-525 bp	1.0	79.0