Hotspot

trnV-UAC-trnM-CAU

LSC · 50654-50835 · 182 bp

Back to Lepechiniella

Pi 0.022
MarkerSeek score 65.10
Primer available yes
Label trnV-UAC-trnM-CAU

Feature Metadata

label_name
trnV-UAC-trnM-CAU
feature_type
igs
region
LSC
length_bp
182
variable_sites
4
pi
0.022
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnV-UAC-trnM-CAU_p1 ACCAACTGAGCTATAGCCCT CCCGCCGTATGAAAGCAATA 50.0 50.0 57.8 58.4 245-246 bp 1.0 76.6
trnV-UAC-trnM-CAU_p2 CGGACTCCCCTGAAAAACAT CCCGCCGTATGAAAGCAATA 50.0 50.0 57.8 58.4 291-292 bp 1.0 76.2
trnV-UAC-trnM-CAU_p3 TATGACGGACTCCCCTGAAA CCCGCCGTATGAAAGCAATA 50.0 50.0 57.8 58.4 296-297 bp 1.0 75.9
trnV-UAC-trnM-CAU_p4 TAGTATGACGGACTCCCCTG CCCGCCGTATGAAAGCAATA 55.0 50.0 57.6 58.4 299-300 bp 1.0 74.9
trnV-UAC-trnM-CAU_p5 ACCAACTGAGCTATAGCCCT CCGCCGTATGAAAGCAATAC 50.0 50.0 57.8 57.3 244-245 bp 1.0 74.5