Hotspot

ndhC-trnV-UAC

LSC · 49433-49998 · 566 bp

Back to Lepechiniella

Pi 0.00677

MarkerSeek score 55.70

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 566
variable_sites: 3
pi: 0.00677
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.78
markerseek_score: 55.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CGGGGCTAAAACTCCTGAAA	CCGAGAAAGTCTACGGTTCG	50.0	55.0	57.8	58.1	589-701 bp	1.0	82.0
ndhC-trnV-UAC_p2	CCTTTGCTAATCGGGGCTAA	CCGAGAAAGTCTACGGTTCG	50.0	55.0	57.7	58.1	600-712 bp	1.0	80.8
ndhC-trnV-UAC_p3	ATCGGGGCTAAAACTCCTGA	CCGAGAAAGTCTACGGTTCG	50.0	55.0	58.4	58.1	591-703 bp	1.0	80.2
ndhC-trnV-UAC_p4	AATCGGGGCTAAAACTCCTG	CCGAGAAAGTCTACGGTTCG	50.0	55.0	57.6	58.1	592-704 bp	1.0	80.1
ndhC-trnV-UAC_p5	CGGGGCTAAAACTCCTGAAA	AGTGCATGTTGGGTCTTTGA	50.0	45.0	57.8	57.6	653-765 bp	1.0	79.9