Hotspot

psaA-ycf3

LSC · 40604-41326 · 723 bp

Back to Lepechiniella

Pi 0.0043

MarkerSeek score 64.60

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 723
variable_sites: 3
pi: 0.0043
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GTTCTTGAAAAATGGCCCGG	ATTGCAGAGGCTTGGTTTGA	50.0	45.0	57.9	58.0	921-944 bp	1.0	81.8
psaA-ycf3_p2	GTTCTTGAAAAATGGCCCGG	TTGCAGAGGCTTGGTTTGAT	50.0	45.0	57.9	58.0	920-943 bp	1.0	81.8
psaA-ycf3_p3	GTTCTTGAAAAATGGCCCGG	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	902-925 bp	1.0	81.5
psaA-ycf3_p4	GTTCTTGAAAAATGGCCCGG	GGAAACAGGCTATAGCGCTT	50.0	50.0	57.9	58.0	884-907 bp	1.0	81.5
psaA-ycf3_p5	TTGAAAAATGGCCCGGTTTG	TTGCAGAGGCTTGGTTTGAT	45.0	45.0	57.8	58.0	916-939 bp	1.0	80.4