Hotspot

rpoB-trnC-GCA

LSC · 26354-27553 · 1200 bp

Back to Lepechiniella

Pi 0.0143

MarkerSeek score 68.20

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1200
variable_sites: 17
pi: 0.0143
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CCTCATTTCCATTACCGAGCA	CCGGATTTGAACTGGGGAAA	47.6	50.0	58.1	58.1	1284-1293 bp	1.0	72.7
rpoB-trnC-GCA_p2	CCTCATTTCCATTACCGAGCA	AAAAGGATTTGCAGTCCCCC	47.6	50.0	58.1	58.4	1266-1275 bp	1.0	70.5
rpoB-trnC-GCA_p3	CCTCATTTCCATTACCGAGC	CCGGATTTGAACTGGGGAAA	50.0	50.0	56.3	58.1	1284-1293 bp	1.0	67.6
rpoB-trnC-GCA_p4	CCTCATTTCCATTACCGAGCA	TTTGTTGATCAGGCGACACC	47.6	50.0	58.1	58.8	1303-1312 bp	1.0	67.2
rpoB-trnC-GCA_p5	CCCTCATTTCCATTACCGAGC	CCGGATTTGAACTGGGGAAA	52.4	50.0	58.8	58.1	1285-1294 bp	1.0	67.1