Hotspot

rps15-ycf1

SSC · 124802-125132 · 331 bp

Back to Lepanthes

Pi 0.0185
MarkerSeek score 64.80
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
331
variable_sites
6
pi
0.0185
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 ACTTGAAATTCAACAGACCCAC ACAGCAGTCGTTTCAGTATGT 40.9 42.9 57.1 57.6 497-505 bp 1.0 47.5
rps15-ycf1_p2 CTTGAAATTCAACAGACCCACT ACAGCAGTCGTTTCAGTATGT 40.9 42.9 56.8 57.6 496-504 bp 1.0 45.3
rps15-ycf1_p3 ACTTGAAATTCAACAGACCCAC AGCAGTCGTTTCAGTATGTCA 40.9 42.9 57.1 57.3 495-503 bp 1.0 45.3
rps15-ycf1_p4 TGAAATTCAACAGACCCACT ACAGCAGTCGTTTCAGTATGT 40.0 42.9 54.8 57.6 494-502 bp 1.0 45.0
rps15-ycf1_p5 TTGAAATTCAACAGACCCACT ACAGCAGTCGTTTCAGTATGT 38.1 42.9 55.6 57.6 495-503 bp 1.0 43.7