Hotspot

ndhF-rpl32

SSC · 113638-114453 · 816 bp

Back to Lepanthes

Pi 0.0253

MarkerSeek score 76.00

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 816
variable_sites: 20
pi: 0.0253
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGGGAGTGGAAAAAGGGGTA	GCTTTTACTGCCTCTAAAGATCC	50.0	43.5	58.2	57.3	944-959 bp	1.0	52.5
ndhF-rpl32_p2	TGGGAGTGGAAAAAGGGGTA	AGAAAAAGCTTTTACTGCCTCT	50.0	36.4	58.2	56.2	951-966 bp	1.0	51.9
ndhF-rpl32_p3	AACTGGGAGTGGAAAAAGGG	GCTTTTACTGCCTCTAAAGATCC	50.0	43.5	57.6	57.3	947-962 bp	1.0	50.8
ndhF-rpl32_p4	AACTGGGAGTGGAAAAAGGG	AGAAAAAGCTTTTACTGCCTCT	50.0	36.4	57.6	56.2	954-969 bp	1.0	50.1
ndhF-rpl32_p5	TGGGAGTGGAAAAAGGGGTA	AAGAAAAAGCTTTTACTGCCTCT	50.0	34.8	58.2	56.9	952-967 bp	1.0	49.5