Hotspot

psbB-psbT

LSC · 73823-74330 · 508 bp

Back to Lepanthes

Pi 0.0142

MarkerSeek score 67.80

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 508
variable_sites: 7
pi: 0.0142
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	GCCAGAACCTTGTTCAGAGA	TGGAACTTTAGGTGGTTCTCG	50.0	47.6	57.4	57.6	700-715 bp	1.0	66.9
psbB-psbT_p2	GCCAGAACCTTGTTCAGAGA	GGAACTTTAGGTGGTTCTCGA	50.0	47.6	57.4	57.3	699-714 bp	1.0	64.7
psbB-psbT_p3	CGCCAGAACCTTGTTCAGAG	TGGAACTTTAGGTGGTTCTCG	55.0	47.6	58.8	57.6	701-716 bp	1.0	64.5
psbB-psbT_p4	GCCAGAACCTTGTTCAGAGAT	TGGAACTTTAGGTGGTTCTCG	47.6	47.6	57.9	57.6	700-715 bp	1.0	62.8
psbB-psbT_p5	CGCCAGAACCTTGTTCAGAG	GGAACTTTAGGTGGTTCTCGA	55.0	47.6	58.8	57.3	700-715 bp	1.0	62.3