Hotspot

accD-psaI

LSC · 57746-58552 · 807 bp

Back to Lepanthes

Pi 0.0496

MarkerSeek score 84.30

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 807
variable_sites: 40
pi: 0.0496
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	CACGGTTTCCTTCCCTTGAA	AAAGAAGCCATTGCGATTGC	50.0	45.0	58.0	58.0	929-959 bp	1.0	83.2
accD-psaI_p2	TTTGACCCAATCGTACCACG	AAAGAAGCCATTGCGATTGC	50.0	45.0	58.2	58.0	989-1019 bp	1.0	81.6
accD-psaI_p3	TCAGCTACACGGTTTCCTTC	AAAGAAGCCATTGCGATTGC	50.0	45.0	57.5	58.0	936-966 bp	1.0	79.8
accD-psaI_p4	CACGGTTTCCTTCCCTTGAA	CGATTGCCGGAAAGACTAGG	50.0	55.0	58.0	58.4	916-946 bp	1.0	79.8
accD-psaI_p5	TGACCCAATCGTACCACGTA	AAAGAAGCCATTGCGATTGC	50.0	45.0	58.5	58.0	987-1017 bp	1.0	79.5