Hotspot

atpB-rbcL

LSC · 52910-53940 · 1031 bp

Back to Lepanthes

Pi 0.0191

MarkerSeek score 64.80

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 1031
variable_sites: 19
pi: 0.0191
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CAGAAGTAGCGGGATTGCTT	ACCAGCTTTAAATCCAACGC	50.0	45.0	58.0	57.0	1078-1104 bp	1.0	75.6
atpB-rbcL_p2	CAGAAGTAGCGGGATTGCTT	AGCTTTAAATCCAACGCTTGC	50.0	42.9	58.0	58.1	1075-1101 bp	1.0	75.3
atpB-rbcL_p3	CAGAAGTAGCGGGATTGCTT	GCTTTAAATCCAACGCTTGCT	50.0	42.9	58.0	58.1	1074-1100 bp	1.0	75.3
atpB-rbcL_p4	TGGGCCAATAATTTGAGCGA	ACCAGCTTTAAATCCAACGC	45.0	45.0	57.9	57.0	1140-1166 bp	1.0	74.6
atpB-rbcL_p5	TGGGCCAATAATTTGAGCGA	GCTTTAAATCCAACGCTTGCT	45.0	42.9	57.9	58.1	1136-1162 bp	1.0	74.2