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petN-psbM

LSC · 28326-29085 · 760 bp

Back to Lepanthes

Pi 0.0212

MarkerSeek score 66.80

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 760
variable_sites: 16
pi: 0.0212
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGGGGACGTCATTTGCATG	ACGTCAAAACAGTCAGCCAA	50.0	45.0	58.0	58.3	896-922 bp	1.0	79.9
petN-psbM_p2	TAGTAAGTCTCGCTTGGGCT	ACGTCAAAACAGTCAGCCAA	50.0	45.0	58.2	58.3	872-898 bp	1.0	79.0
petN-psbM_p3	TAGGGGACGTCATTTGCATG	GCGCTTATTGCTACTACGCT	50.0	50.0	58.0	58.5	954-980 bp	1.0	77.7
petN-psbM_p4	GATAGGGGACGTCATTTGCA	ACGTCAAAACAGTCAGCCAA	50.0	45.0	57.7	58.3	898-924 bp	1.0	77.6
petN-psbM_p5	TAGGGGACGTCATTTGCATG	TACGTCAAAACAGTCAGCCA	50.0	45.0	58.0	57.4	897-923 bp	1.0	77.1