Hotspot

trnK-UUU-rps16

LSC · 4194-4745 · 552 bp

Back to Lepanthes

Pi 0.028
MarkerSeek score 62.00
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
552
variable_sites
15
pi
0.028
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT TCGATCCCGAAGAGAAGGAA 50.0 50.0 58.2 57.9 787-800 bp 1.0 81.1
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT TCGATCCCGAAGAGAAGGAA 50.0 50.0 58.2 57.9 786-799 bp 1.0 81.1
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT TCTATCCCAATGGGCCATCT 50.0 50.0 58.2 57.9 829-842 bp 1.0 80.9
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT TCTATCCCAATGGGCCATCT 50.0 50.0 58.2 57.9 828-841 bp 1.0 80.9
trnK-UUU-rps16_p5 CCTTTCAGGATCAGTCGCAG TCTATCCCAATGGGCCATCT 55.0 50.0 58.4 57.9 920-933 bp 1.0 79.1