Hotspot

ycf1

SSC · 121142-126676 · 5535 bp

Back to Leonurus

Pi 0.0012

MarkerSeek score 53.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5535
variable_sites: 10
pi: 0.0012
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCCAGATTGGATATGGGATGC	AGTCGATATTGAGGCCTGGA	47.6	50.0	57.3	57.9	204 bp	1.0	66.7
ycf1_p2	CCAGATTGGATATGGGATGCT	AGTCGATATTGAGGCCTGGA	47.6	50.0	57.3	57.9	203 bp	1.0	66.6
ycf1_p3	CCAGATTGGATATGGGATGCTT	AGTCGATATTGAGGCCTGGA	45.5	50.0	58.0	57.9	203 bp	1.0	64.1
ycf1_p4	CCAGATTGGATATGGGATGC	AGTCGATATTGAGGCCTGGA	50.0	50.0	55.7	57.9	203 bp	1.0	62.1
ycf1_p5	TCCAGATTGGATATGGGATGC	AAGTCGATATTGAGGCCTGG	47.6	50.0	57.3	57.1	205 bp	1.0	60.0